Volume 3, Issue 1, June 2019, Page: 7-17
Evaluation of Bioactive Compounds in Pomegranate Fruit Parts as an Attempt for Their Application as an Active Edible Film
Osama Mohamed Mabrouk, Department of Horticulture Crops Processing, Food Technology Research Institute, ARC, Giza, Egypt
Omayma El-Sayed Shaltout, Food Science Department, Faculty of Agriculture, Saba-Basha, Alexandria University, Alexandria, Egypt
Wafaa Aly Amin, Department of Horticulture Crops Processing, Food Technology Research Institute, ARC, Giza, Egypt
Thanaa Mustafa Ezz, Plant Production Department, Faculty of Agriculture, Saba-Basha, Alexandria University, Alexandria, Egypt
Ahmed Mohamed Zeitoun, Food Science Department, Faculty of Agriculture, Saba-Basha, Alexandria University, Alexandria, Egypt
Received: Apr. 21, 2019;       Accepted: May 30, 2019;       Published: Jun. 12, 2019
DOI: 10.11648/j.jb.20190301.12      View  51      Downloads  36
Pomegranate fruit contains high content of phytochemical constituents which have many health benefits. Peel and rind are wastes of pomegranate fruit processing, represent up to 50% of its weight. This study aimed to evaluate the ethanolic extract of pomegranate fruit parts: arils, rind and peel as sources of bioactive compounds as well as their antioxidant and antimicrobial activities for their application as an active edible film. Results clearly demonstrated that peel extract (PE) had the highest content of total phenolics and flavonoids (342 mg GAE /g and 82.33mg catechol /g, respectively ) followed by rind extract (RE) containing 213.00 mg GAE/g and 70.50 mg catechol /g, respectively, and finally arils extract (AE) ( 108.22 mg GAE /g and 55.58 mg catechol/g), respectively. Results indicated that total anthocyanins content was concentrated in PE (15.24mg Cynidian-3-glycoside/g) and AE (11.04 mg Cynidian-3-glycoside/g), while RE (6.51 mg Cynidian-3-glycoside/g) had the lowest value. Peel extract exhibited the highest antioxidant activity followed by RE and were significantly higher than that of AE. These results were confirmed with the DPPH and ABTS+ assays. Consequently, PE followed by RE had higher antimicrobial activity against several pathogenic strains than AE and can be used as natural preservative for food. Peel extract and RE were incorporated into pectin film at concentration of 15 mg/ml to develop an active edible film. Pectin film without the tested fruit parts extract was used as the control film. The obtained results revealed that the film prepared from pectin with PE and RE was successfully developed and considers as an active edible film with antioxidant and antimicrobial properties.
Pomegranate, Ethanolic Extract, Antioxidant and Antimicrobial Activity, Active Edible Film
To cite this article
Osama Mohamed Mabrouk, Omayma El-Sayed Shaltout, Wafaa Aly Amin, Thanaa Mustafa Ezz, Ahmed Mohamed Zeitoun, Evaluation of Bioactive Compounds in Pomegranate Fruit Parts as an Attempt for Their Application as an Active Edible Film, Journal of Biomaterials. Vol. 3, No. 1, 2019, pp. 7-17. doi: 10.11648/j.jb.20190301.12
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Karimi, M., Sadeghi, R., & Kokini, J. (2017). Pomegranate as a promising opportunity in medicine and nanotechnology. Trends in Food Science and Technology, 69: 59–73.
Hamouda, H. A., Ibrahim, G. E., & Hafez, O. M. (2014). Nutritional status, fruit quality and volatile compounds in eight Egyptian pomegranate cultivars. British Journal of Applied Science & Technology, 4 (22): 3263-3280.
Ambigaipalan, P., de Camargo, A. C., & Shahidi, F. (2016). Phenolic compounds of pomegranate byproducts (outer skin, mesocarp, divider membrane) and their antioxidant activities. Journal of agricultural and food chemistry, 64 (34): 6584-6604.
Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of ethnopharmacology, 109 (2): 177-206.
Pagliarulo, C., De Vito, V., Picariello, G., Colicchio, R., Pastore, G., Salvatore, P., & Volpe, M. G. (2016). Inhibitory effect of pomegranate (Punica granatum L.) Polyphenol extracts on the bacterial growth and survival of clinical isolates of pathogenic Staphylococcus aureus and Escherichia coli. Food Chemistry, 190: 824-831.
Orgil, O., Schwartz, E., Baruch, L., Matityahu, I., Mahajna, J., & Amir, R.(2014). The Antioxidative and anti-proliferative potential of non-edible organs of the pomegranate fruit and tree. LWT-Food Science and Technology, 58(2): 571–577.
Akhtar, S., Ismail, T., Fraternale, D., & Sestili, P. (2015). Pomegranate peel and peel extracts: Chemistry and food features. Food Chemistry, 174: 417– 425.
Abid, M., Yaich, H., Cheikhrouhou, S., Khemakhem, I., Bouaziz, M., Attia, H., & Ayadi, M. A. (2017). Antioxidant properties and phenolic profile characterization by LCMS/MS of selected Tunisian pomegranate peels. Journal of Food Science and Technology, 54 (9): 2890–2901.
Gullon, B., Pintado, M. E., &Viuda-Martos, M. (2016). Assessment of polyphenolic profile and Antibacterial activity of pomegranate peel (Punica granatum) flour obtained from coproduct of juice extraction. Food Control, 59: 94–98.
Goula, A. M., & Lazarides, H. N. (2015). Integrated processes can turn industrial food waste into valuable food by-products and/or ingredients: The cases of olive mill and pomegranate wastes. Journal of Food Engineering, 167: 45-50.
Hasnaoui, N., Wathelet, B., & Jiménez-Araujo, A. (2014). Valorization of pomegranate peel from 12 cultivars: dietary fibre composition, antioxidant capacity and functional properties. Food Chem., 160: 196–203.
Mphahlele, R. R., Fawole, O. A., Makunga, N. P., & Opara, U. L. (2016). Effect of drying on the bioactive compounds, antioxidant, antibacterial and antityrosinase activities of pomegranate peel. BMC complementary and alternative medicine, 16 (1): 143.
Al-Zoreky, N. S. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International Journal of Food Microbiology, 134 (3): 244–248.
Oriani, V. B., Molina, G., Chiumarelli, M., Pastore, G. M., & Hubinger, M. D. (2014). Properties of cassava starch‐based edible coating containing essential oils. Journal of food science, 79 (2): 189-194.
Wrona, M., Bentayeb, K., & Nerin, C. (2015). A novel active packaging for extending the shel –life of fresh mushroom (Agaricuss bisporus). Food Control, 54: 200-207.
Realini, C. E., & Marcos, B. (2014). Active and intelligent packaging systems for a modern society. Meat Science, 98 (3): 404-419.
Norajit, K., Kim, K. M., & Ryu, G. H. (2010). Comparative studies on the characterization and antioxidant properties of biodegradable alginate films containing ginseng extract. Journal of Food Engineering, 98 (3): 377-384.
Abirami, A., Nagarani, G., & Siddhuraju, P. (2014). In vitro antioxidant, antidiabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits. Food Science and Human Wellness, 3: 16–25.
Barros, L., Cabrita, L., Boas, M. V., Carvalho, A. M., & Ferreira, I. C. F. R. (2011). Chemical, biochemical and electrochemical assays to evaluate phytochemicals and antioxidant activity of wild plants. Food Chemistry, 127: 1600-1608.
Elfalleh, W., Tlili, N., Nasri, N., Yahia, Y., Hannachi, H., Chaira, N., & Ferchichi, A. (2011). Antioxidant capacities of phenolic compounds and tocopherols from Tunisian pomegranate (Punica granatum) fruits. Journal of food science, 76 (5): 707-713.
Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28 (1): 25-30.
Hwang, E. S., & Do Thi, N. (2014). Effects of extraction and processing methods on antioxidant compound contents and radical scavenging activities of laver (Porphyra tenera). Preventive nutrition and food science, 19 (1): 40 - 48.
Hamad, G. M., Darwish, A. M., Abu-Serie, M. M., & El Sohaimy, S. A. (2017). Antimicrobial, antioxidant and anti-inflammatory characteristics of combination (Cassia fistula and Ocimum basilicum) extract as natural preservative to control & prevent food contamination. J. Food Nutr. Res, 5 (10): 771-780.‏
Kadaikunnan, S., Rejiniemon, T. S., Khaled, J. M., Alharbi, N. S., & Mothana, R. (2015). In-vitro antibacterial, antifungal, antioxidant and functional properties of Bacillus amyloliquefaciens. Annals of clinical microbiology and antimicrobials, 14 (1): 9.
SAS (2004). SAS Procedure Guide "Version 6.12 Ed." SAS Institute Inc., Cary.
Orak, H. H., Yagar, H., Isbilir, S. S. (2012). Comparison of antioxidant activities of juice, peel, and seed of pomegranate ( Punica granatum L) and inter-relationships with total phenolic, tannin, anthocyanin, and flavonoid contents. Food Science and Biotechnology, 21: 373-387.
Derakhshan, Z., Ferrante, M., Tadi, M., Ansari, F., Heydari, A., Hosseini, M. S., & Sadrabad, E. K. (2018). Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food and chemical toxicology, 114: 108-111.
Rosas‐Burgos, E. C., Burgos‐Hernández, A., Noguera‐Artiaga, L., Kačániová, M., Hernández‐García, F., Cárdenas‐López, J. L., & Carbonell‐Barrachina, Á. A. (2017). Antimicrobial activity of pomegranate peel extracts as affected by cultivar. Journal of the Science of Food and Agriculture, 97(3): 802-810.
Sumaiya, K., Jahurul, M. H. A., & Zzaman, W. (2018). Evaluation of biochemical and bioactive properties of native and imported pomegranate (Punica granatum L.) cultivars found in Bangladesh. International Food Research Journal, 25 (2): 737-746.
Farag, R., Abdelatif, M., Emam, S., & Tawfeek, L. (2014). Phytochemical screening and polyphenol constituents of pomegranate peels and leave juices. Agr Soil Sci., 1 (6): 86- 93.
Souleman, A. M., & Ibrahim, G. E. (2016). Evaluation of Egyptian pomegranate cultivars for antioxidant activity, phenolic and flavonoid contents. Egyptian Pharmaceutical Journal, 15 (3): 143.
Zhu, F. Z., Yuan, Z. H., Zhao, X. Q., Yin, Y. L., & Feng, L. J. (2015). Composition and contents of anthocyanins in different pomegranate cultivars. Acta Hortic, 1089: 35-41.
Hou, Z., Qin, P., Zhang, Y., Cui, S., and Ren, G. (2013). Identification of anthocyanins isolated from black rice (Oryza sativa L.) and their degradation kinetics. Food Research International, 50: 691-697.
Ibrahium, M. I. (2010). Efficiency of pomegranate peel extract as antimicrobial, antioxidant and protective agents. World Journal of Agricultural Sciences, 6 (4): 338-344.
Cristofori, V., Caruso, D., Latini, G., Dell’Agli, M., Cammilli, C., Rugini, E., Bignami C., Muleo, R.. (2011). Fruit quality of Italian pomegranate (Punica granatum L.) autochthonous varieties. European Food Research and Technology, 232 (3): 397–403.
Zaki, S. A., Abdelatif, S. H., Abdelmohsen, N. R., & Ismail, F. A. (2015). Phenolic Compounds and Antioxidant Activities of Pomegranate Peels. International Journal of Food Engineering, 1 (2).‏
Yan, L., Zhou, X., Shi, L., Shalimu, D., Ma, C., & Liu, Y. (2017). Phenolic profiles and antioxidant activities of six Chinese pomegranate (Punica granatum L.) cultivars. International journal of food properties, 20(1): 94-107.
Jalal, H., Pal, M. A., Hamdani, H., Rovida, M., & Khan, N. N. (2018). Antioxidant activity of pomegranate peel and seed powder extracts. Journal of Pharmacognosy and Phytochemistry, 7 (5): 992-997.‏
Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food chemistry, 96 (2): 254-260.‏
Dahham, S. S., Ali, M. N., Tabassum, H., & Khan, M. (2010). Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). American-Eurasian Journal of Agricultural & Environmental Sciences, 9 (3): 273-281.
Nuamsetti, T., Dechayuenyong, P., & Tantipaibulvut, S. (2012). Antibacterial activity of pomegranate fruit peels and arils. Science Asia, 38 (3): 319-22.
Khan, J. A., & Hanee, S. (2011). Antibacterial properties of Punica granatum peels. International Journal of Applied Biology and Pharmaceutical Technology, 2 (3): 23-27.
Anibal, P. C., Peixoto, I. T. A., Foglio, M. A., & Höfling, J. F. (2013). Antifungal activity of the ethanolic extracts of (Punica granatum L.) and evaluation of the morphological and structural modifications of its compounds upon the cells of Candida spp. Brazilian Journal of Microbiology, 44 (3): 839-848.
Bassiri-Jahromi, S. (2018). In vivo comparative evaluation of the pomegranate (Punica granatum) peels extract as an alternative agent to nystatin against oral candidiasis. Iranian journal of medical sciences, 43 (3): 296.
Ali, A., Chen, Y., Liu, H., Yu, L., Baloch, Z., Khalid, S., & Chen, L. (2019). Starch-based antimicrobial films functionalized by pomegranate peel. International journal of biological macromolecules, 129 (15): 1120-1126.
Duman, A., Ozgen, M., Dayisoylu, K., Erbil, N., & Durgac, C. (2009). Antimicrobial activity of six pomegranate (Punica granatum L.) varieties and their relation to some of their pomological and phytonutrient characteristics. Molecules, 14 (5): 1808-1817.
Eça, K. S., Machado, M. T., Hubinger, M. D., & Menegalli, F. C. (2015). Development of active films from pectin and fruit extracts: Light protection, antioxidant capacity, and compounds stability. Journal of food science, 80 (11): 2389-2396.
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